The Role of Toll-like Receptor-4 in Periodontitis Patients With End-stage Renal Disease in a Sample of Egyptian Population
Status: Recruiting
Location: See location...
Study Type: Observational
SUMMARY
Chronic kidney disease (CKD) is a significant public health issue in Egypt, leading to end-stage renal disease (ESRD). ESRD patients often suffer from oral abnormalities due to the disease and medications. Periodontitis is linked to systemic diseases, including renal disease, with inflammation playing a key role. TLR4 genetic variations may influence susceptibility to CKD and periodontitis. This study investigates the association between TLR4 \[Gly/Thr (rs2149356)\] polymorphism and chronic periodontal disease in ESRD patients in Egypt.
Eligibility
Participation Requirements
Sex: All
Minimum Age: 30
Maximum Age: 60
Healthy Volunteers: t
View:
• Age: 30 to 60 years
• Gender: Both male and female
• Diagnosed with Periodontitis stage II and/or stage III
• Systemically healthy, no history of systemic diseases
• Not on long-term medications
• Age: 30 to 60 years
• Gender: Both male and female
• Diagnosed with end-stage renal disease (ESRD) on regular hemodialysis
• Diagnosed with Periodontitis stage II and/or stage III
• Unrelated Egyptians from a similar socioeconomic status, residing in the same geographical area
• Underlying type 2 diabetes mellitus
Locations
Other Locations
Egypt
hemodialysis center at Benha University Hospital.
RECRUITING
Al Fayyum
Contact Information
Primary
Asmaa Bakr elshaf3y, PHD
Asmaa.AbdAlRaouf@gu.edu.eg
01000093885
Time Frame
Start Date:2025-04-01
Estimated Completion Date:2025-08-01
Participants
Target number of participants:100
Treatments
Healthy Subjects with Periodontitis
Description: This group consists of 50 systemically healthy individuals diagnosed with periodontitis. Participants are of both genders, aged between 30 and 60 years. They have Periodontitis stage II and/or stage III and do not have any history of systemic diseases or long-term medication use. All smokers were excluded from this group.~Interventions of Interest:~Clinical assessment of periodontal parameters such as Plaque Index (PI), Gingival Index (GI), Probing Depth (PD), and Clinical Attachment Level (CAL) using standard methods.~Evaluation of the proportion of bleeding sites as a Bleeding on Probing (BOP) score.
ESRD Patients with Periodontitis
Description: This group includes 50 individuals diagnosed with end-stage renal disease (ESRD) who are on regular hemodialysis. Participants are of both genders, aged between 30 and 60 years, and unrelated Egyptians from similar socioeconomic statuses residing in the same geographical area. They also suffer from Periodontitis stage II and/or stage III. All participants have underlying type 2 diabetes mellitus. Exclusions include those with kidney transplants, other systemic diseases except diabetes, history of malignancy, and smokers.~Interventions of Interest:~Clinical assessment of periodontal parameters similar to Group I, including PI, GI, PD, and CAL.~Analysis of genetic variations in the TLR4 gene through blood sample collection and DNA extraction.~SNP genotyping in the TLR4 gene performed using Real Time Polymerase Chain Reaction (RT-PCR).